Sequential density fractionation across soils of contrasting mineralogy: evidence for both microbial- and mineralcontrolled soil organic matter stabilization
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Author(s)
Sollins, Phillip
Kramer, Marc G.
Swanston, Christopher
Lajtha, Kate
Filley, Timothy R.
Aufdenkampe, Anthony K.
Wagai, Rota
Bowden, Richard D.
Date Issued
2009
Abstract
Sequential density fractionation separated soil particles into “light” predominantly mineral-free organic matter vs. increasingly “heavy” organo-mineral particles in four soils of widely differing mineralogy. With increasing particle density C concentration decreased, implying that the soil organic matter (OM) accumulations were thinner. With thinner accumulations we saw evidence for both an increase in 14C-based mean residence time (MRT) of the OM and a shift from plant to microbial origin.Evidence for the latter included: (1) a decrease in C/N, (2) a decrease in lignin phenols and an increase in their oxidation state, and (3) an increase in δ13C and δ15N. Although bulk-soil OM levels varied substantially across the four soils, trends in OM composition and MRT across the density fractions were similar. In the intermediate density fractions (~1.8–2.6 g cm−3), most of the reactive sites available for interaction with organic molecules were provided by aluminosilicate clays, and OM characteristics were consistent with a layered mode of OM accumulation. With increasing density (lower OM loading) within this range, OM showed evidence of an increasingly microbial origin. We hypothesize that this microbially derived OM was young at the time of attachment to the mineral surfaces but that it persisted due to both binding with mineral surfaces and protection beneath layers of younger, less microbially processed C. As a result of these processes, the OM increased in MRT, oxidation state, and degree of microbial processing in the sequentially denser intermediate fractions. Thus mineral surface chemistry is assumed to play little role in determining OM composition in these intermediate fractions. As the separation density was increased beyond ~2.6 g cm−3, mineralogy shifted markedly: aluminosilicate clays gave way first to light primary minerals including quartz, then at even higher densities to various Fe-bearing primary minerals. Correspondingly, we observed a marked drop in δ15N, a weaker decrease in extent of microbial processing of lignin phenols, and some evidence of a rise in C/N ratio. At the same time, however, 14C-based MRT time continued its increase. The increase in MRT, despite decreases in degree of microbial alteration, suggests that mineral surface composition (especially Fe concentration) plays a strong role in determining OM composition across these two densest fractions.
Journal
Biogeochemistry
Department
Biology
Citation
Sollins, P., Kramer, M.G., Swanston, C. (2009). Sequential density fractionation across soils of contrasting mineralogy: evidence for both microbial- and mineral controlled soil organic matter stabilization. Biogeochemistry, 96(1-3): 209-231. doi: 10.1007/s10533-009-9359-z
Publisher
Springer Science + Business Media B.V.
Version of Article
Published article
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DOI
10.1007/s10533-009-9359-z
ISSN
e1573-515X
0168-2563
Rights
This article is published by Springer Netherlands in Biogeochemistry (2009) Sollins, et al. All rights reserved.
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