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dc.contributor.authorMcCullagh, Bonnie S.
dc.contributor.authorWissinger, Scott A.
dc.contributor.authorMarcus, Jeffrey M.
dc.date.accessioned2016-03-23T19:31:03Z
dc.date.available2016-03-23T19:31:03Z
dc.date.issued2015-10-10
dc.identifier.citationMcCullagh, Bonnie S., Scott A. Wissinger, and Jeffrey M. Marcus. 2015. "Identifying PCR primers to facilitate molecular phylogenetics in Caddisflies (Trichoptera)." Zoological Systematics 40, no. 4: 459-469.en_US
dc.identifier.issn1000-0739
dc.identifier.urihttp://hdl.handle.net/10456/41192
dc.description.abstractThe molecular phylogenetics of the Lepidoptera (butterflies and moths) is well studied, but that of Trichoptera (caddisflies), the sister clade of Lepidoptera, is less studied. The PCR primer libraries developed for lepidopteran phylogenetics might work in Trichoptera. DNA from 8 caddisfly species (Asynarchus nigriculus (Banks, 1908), Grammotaulius lorettae Denning, 1941, Hesperophylax occidentalis (Banks, 1908), Limnephilus externus Hagen, 1861, Limnephilus picturatus McLachlan, 1875, Limnephilus secludens Banks, 1914, Limnephilus sublunatus Provancher, 1877 and Agrypnia deflata (Milne, 1931)) was used to screen for amplification. 107 primer pairs for 45 nuclear and 3 mitochondrial genes were tested. Primers for 1 new gene (40S ribosomal protein S2 (RPS2)) and 8 genes previously used in Trichopteran phylogenetics were recovered (16S rRNA, 18S rRNA, carbamoyl-phosphate synthetase (CAD), cytochrome oxidase I (COI), cytochrome oxidase II (COII), elongation factor-1 alpha (EF-1 alpha), isocitrate dehydrogenase (IDH), and RNA polymerase-II (POL-II)). New primer pairs extended the genomic region sampled for many genes. Evolution rates among loci varied by 2 orders of magnitude. Differences among evolution rates and modes of inheritance offer flexible tools for resolving phylogenetic questions and examining genome evolution in the Trichoptera. Screening libraries of PCR primers is a useful approach for identifying PCR primers in related taxa with limited molecular genetic resources.en_US
dc.language.isoen_USen_US
dc.publisherInstitute of Zoology, Chinese Academy of Sciencesen_US
dc.relation.ispartofZoological Systematicsen_US
dc.relation.isversionofhttp://www.zootax.com.cn/viewmulu_en.aspx?qi_id=1508&mid=47044en_US
dc.subjectTrichopteraen_US
dc.subjectmolecular phylogeneticsen_US
dc.subjectmosaic genome evolutionen_US
dc.subjectrates of sequence evolutionen_US
dc.subjectPCR primer libraryen_US
dc.titleIdentifying PCR primers to facilitate molecular phylogenetics in Caddisflies (Trichoptera)en_US
dc.description.versionPublished articleen_US
dc.contributor.departmentBiologyen_US
dc.citation.volume40en_US
dc.citation.issue4en_US
dc.citation.spage459en_US
dc.citation.epage469en_US
dc.identifier.doi10.11865/zs.20150404
dc.contributor.avlauthorWissinger, Scott A.


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