The antibacterial agent, triclosan, has been incorporated into hand soaps for decades and is also found in other household products, such as plastics and clothing. While hand soaps do not contain enough triclosan to kill most bacteria, they may select for triclosan resistance and for cross-resistance to clinically relevant antibiotics. Pseudomonas aeruginosa, a bacterial species that is the leading cause of death from hospital-acquired infections, is famous for displaying high levels of antibiotic resistance as well as the ability to thrive in diverse environments. I hypothesized that P. aeruginosa populations in restrooms stocked with soap containing triclosan harbor a larger percentage of P. aeruginosa cells resistant to triclosan than populations in restrooms stocked with triclosan-free soap. To test this hypothesis, swab samples from soap dispensers, faucets, door knobs, and light switches were incubated in soap solutions with or without triclosan and then plated on Pseudomonas-selection agar to assess colony forming units. Exposure time to triclosan required to differentiate resistant from non-resistant strains was determined by using the resistant strain PAO1. While selection of triclosan-resistant strains of P. aeruginosa has been previously demonstrated for soap dispensers in hospital settings, demonstrating similar selection mechanisms in a non-clinical environment strongly supports a role for antibiotic soaps in the selection of antibiotic resistant strains in the population at large. It was found that Psuedomonas environmental isolates could survive plain soap treatment after having been previously exposed to triclosan-containing soap. This implies that bacterial populations are more resilient and more difficult to kill off with exposure to triclosan soap and provides added impetus for discontinuing the indiscriminate use of this antibacterial agent.