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dc.contributor.authorHolch, Anne
dc.contributor.authorWebb, Kristen
dc.contributor.authorLukjancenko, Oksana
dc.contributor.authorUssery, David
dc.contributor.authorRosenthal, Benjamin M.
dc.contributor.authorGram, Lone
dc.date.accessioned2018-03-01T13:43:20Z
dc.date.available2018-03-01T13:43:20Z
dc.date.issued2013-05
dc.identifier.citationGenome sequencing identifies two nearly unchanged strains of persistent Listeria monocytogenes isolated in two different fish processing plants sampled six years apart. Applied and Environmental Microbiology, 79(9): 2944-2951. doi: 10.1128/AEM03715-12en_US
dc.identifier.issn0099-2240
dc.identifier.issne1098-5336
dc.identifier.urihttp://hdl.handle.net/10456/45793
dc.description.abstractListeria monocytogenes is a food-borne human-pathogenic bacterium that can cause infections with a high mortality rate. It has a remarkable ability to persist in food processing facilities. Here we report the genome sequences for two L. monocytogenes strains (N53-1 and La111) that were isolated 6 years apart from two different Danish fish processers. Both strains are of serotype 1/2a and belong to a highly persistent DNA subtype (random amplified polymorphic DNA [RAPD] type 9). We demonstrate using in silico analyses that both strains belong to the multilocus sequence typing (MLST) type ST121 that has been isolated as a persistent subtype in several European countries. The purpose of this study was to use genome analyses to identify genes or proteins that could contribute to persistence. In a genome comparison, the two persistent strains were extremely similar and collectively differed from the reference lineage II strain, EGD-e. Also, they differed markedly from a lineage I strain (F2365). On the proteome level, the two strains were almost identical, with a predicted protein homology of 99.94%, differing at only 2 proteins. No single-nucleotide polymorphism (SNP) differences were seen between the two strains; in contrast, N53-1 and La111 differed from the EGD-e reference strain by 3,942 and 3,471 SNPs, respectively. We included a persistent L. monocytogenes strain from the United States (F6854) in our comparisons. Compared to nonpersistent strains, all three persistent strains were distinguished by two genome deletions: one, of 2,472 bp, typically contains the gene for inlF, and the other, of 3,017 bp, includes three genes potentially related to bacteriocin production and transport (lmo2774, lmo2775, and the 3=-terminal part of lmo2776). Further studies of highly persistent strains are required to determine if the absence of these genes promotes persistence. While the genome comparison did not point to a clear physiological explanation of the persistent phenotype, the remarkable similarity between the two strains indicates that subtypes with specific traits are selected for in the food processing environment and that particular genetic and physiological factors are responsible for the persistent phenotype.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofApplied and Environmental Microbiologyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1128/AEM.03715-12en_US
dc.rightsThis article was selected and published in Applied and Environmental Microbiology ©2013 Holch, et al. All rights reserved.en_US
dc.subjectListeria monocytogenesen_US
dc.subjectN53-1en_US
dc.subjectLa111en_US
dc.subjectbacteriaen_US
dc.subjectfood-borne bacteriumen_US
dc.titleGenome sequencing identifies two nearly unchanged strains of persistent Listeria monocytogenes isolated in two different fish processing plants sampled six years aparten_US
dc.description.versionPublished articleen_US
dc.contributor.departmentBiologyen_US
dc.citation.volume79en_US
dc.citation.issue9en_US
dc.citation.spage2944en_US
dc.citation.epage2951en_US
dc.identifier.doi10.1128/AEM03715-12
dc.contributor.avlauthorWebb, Kristen


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