Multiple Class I and Class II Haemophilus ducreyi Strains Cause Cutaneous Ulcers in Children on an Endemic Island

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dc.citation.epage1774en_US
dc.citation.issue11en_US
dc.citation.spage1768en_US
dc.citation.volume67en_US
dc.contributor.authorGrant, Jacob
dc.contributor.authorGonzález-Beiras, Camila
dc.contributor.authorAmick, Kristen
dc.contributor.authorFortney, Kate R.
dc.contributor.authorGangaiah, Dharanesh
dc.contributor.authorHumphreys, Tricia L.
dc.contributor.authorMitjà, Oriol
dc.contributor.authorAbecasis, Ana
dc.contributor.authorSpinola, Stanley M.
dc.contributor.avlauthorHumphreys, Tricia L.
dc.contributor.departmentBiologyen_US
dc.date.accessioned2019-02-12T21:31:35Z
dc.date.available2019-02-12T21:31:35Z
dc.date.issued2018-04-20
dc.description.abstract"Background Together with Treponema pallidum subspecies pertenue, Haemophilus ducreyi is a major cause of exudative cutaneous ulcers (CUs) in children. For H. ducreyi, both class I and class II strains, asymptomatic colonization, and environmental reservoirs have been found in endemic regions, but the epidemiology of this infection is unknown. Methods Based on published whole-genome sequences of H. ducreyi CU strains, a single-locus typing system was developed and applied to H. ducreyi–positive CU samples obtained prior to, 1 year after, and 2 years after the initiation of a mass drug administration campaign to eradicate CU on Lihir Island in Papua New Guinea. DNA from the CU samples was amplified with class I and class II dsrA-specific primers and sequenced; the samples were classified into dsrA types, which were geospatially mapped. Selection pressure analysis was performed on the dsrA sequences. Results Thirty-seven samples contained class I sequences, 27 contained class II sequences, and 13 contained both. There were 5 class I and 4 class II types circulating on the island; 3 types accounted for approximately 87% of the strains. The composition and geospatial distribution of the types varied little over time and there was no evidence of selection pressure. Conclusions Multiple strains of H. ducreyi cause CU on an endemic island and coinfections are common. In contrast to recent findings with T. pallidum pertenue, strain composition is not affected by antibiotic pressure, consistent with environmental reservoirs of H. ducreyi. Such reservoirs must be addressed to achieve eradication of H. ducreyi."en_US
dc.description.versionPublished articleen_US
dc.identifier.citationJacob C Grant, Camila González-Beiras, Kristen M Amick, Kate R Fortney, Dharanesh Gangaiah, Tricia L Humphreys, Oriol Mitjà, Ana Abecasis, Stanley M Spinola; Multiple Class I and Class II Haemophilus ducreyi Strains Cause Cutaneous Ulcers in Children on an Endemic Island, Clinical Infectious Diseases, Volume 67, Issue 11, 13 November 2018, Pages 1768–1774, https://doi.org/10.1093/cid/ciy343en_US
dc.identifier.doi10.1093/cid/ciy343
dc.identifier.issn1058-4838
dc.identifier.issn1537-6591
dc.identifier.urihttp://hdl.handle.net/10456/47913
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofClinical Infectious Diseasesen_US
dc.relation.isversionofhttps://academic.oup.com/cid/article-abstract/67/11/1768/4979512en_US
dc.rights© The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.en_US
dc.subjectHaemophilus ducreyien_US
dc.subjectcutaneous ulcersen_US
dc.subjectsingle-locus typingen_US
dc.subjectmolecular epidemiologyen_US
dc.titleMultiple Class I and Class II Haemophilus ducreyi Strains Cause Cutaneous Ulcers in Children on an Endemic Islanden_US
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