Forensic informativity of ~3,000 bp of coding sequence of domestic dog mtDNA.

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2014-07
Authors
Angleby, Helen
Oskarsson, Mattias
Pang, Junfeng
Zhang, Ya-ping
Leitmer, Thomas
Braham, Caitlyn
Arvestad, Lars
Lundeberg, Joakim
Webb, Kristen
Savolainen, Peter
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Keywords
forensic science , domestic dog , mitochondrial DNA , coding region , control region , exclusion capacity
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Abstract
The discriminatory power of the noncoding control region (CR) of domestic dog mitochondrial DNA alone is relatively low. The extent to which the discriminatory power could be increased by analyzing additional highly variable coding regions of the mitochondrial genome (mtGenome) was therefore investigated. Genetic variability across the mtGenome was evaluated by phylogenetic analysis, and the three most variable ~1 kb coding regions identified. We then sampled 100 Swedish dogs to represent breeds in accordance with their frequency in the Swedish population. A previously published dataset of 59 dog mtGenomes collected in the United States was also analyzed. Inclusion of the three coding regions increased the exclusion capacity considerably for the Swedish sample, from 0.920 for the CR alone to 0.964 for all four regions. The number of mtDNA types among all 159 dogs increased from 41 to 72, the four most frequent CR haplotypes being resolved into 22 different haplotypes.
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Biology
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This article was selected and published in the Journal of Forensic Sciences ©2014 Angleby, et al. All rights reserved.
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Angleby, H., Oskarsson, M., Pang, J., et al. (2014). Forensic informativity of ~3,000 bp of coding sequence of domestic dog mtDNA. Journal of Forensic Sciences, 59(4): 898-908. doi: 10.1111/1556-4029.12504.
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Published article
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Wiley Online Library
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