Prednisone mediated Apoptosis on Human Neuroblastoma Cells

Project Author
Issue Date
2023-12-20
Authors
Dennard, Andrew
Loading...
Thumbnail Image
Embargo
First Reader
Kadmiel, Mahita
Additional Readers
Nelson, Margaret K.
Keywords
Neuroblastoma , Apoptosis , Cells
item.page.distribution
Abstract
Glucocorticoids are commonly prescribed anti-inflammatory synthetic steroid hormones that are used to treat a wide variety of ailments. In many chemotherapy treatments they are included for their anti-inflammatory properties which are useful in regards to reducing the symptoms of other drugs included in chemotherapy treatments. Prednisone is one of these glucocorticoids that is often used for chemotherapy symptom reduction. Prednisone has also been found to have apoptotic inducing properties in some cancer cell lines. Human neuroblastoma (SH-SY5Y) is a type of cancer found in the nervous system that is often prevalent in children under the age of 5 years old. Other glucocorticoids such as dexamethasone have been shown to induce apoptosis in this cell line, but often come with unintended side effects such as the degeneration of neural cells. Prednisone having been already used in chemotherapy treatments is hypothesized to be a viable treatment for the induction of apoptosis in human neuroblastoma cells through the upregulation of pro-apoptotic genes and the down regulation of pro-survival genes Flow Cytometry will be used to test for apoptosis in the prednisone treated neuroblastoma cells in order to determine whether the treatment has resulted in the hypothesized outcome. qRT-PCR will also be performed to explore the pathway of apoptosis induction through a pro-survival gene Bcl-2 as well as a pro-apoptotic gene Bax. The expected results of both of these experiments are that a prednisone treatment of neuroblastoma cells will result in increased apoptosis among them as an upregulation of the chosen pro-survival gene and a downregulation of the chosen pro-apoptotic gene. With the expected results this research will be able to contribute to the growing data showing that the use of glucocorticoids as cancer-killing agents is a viable route of treatment for patients who are already receiving glucocorticoids to mitigate chemotherapy symptoms.
Description
Chair
Major
Biology
Department
Biology
Recorder
License
Citation
Version
Honors
Publisher
Series