Genetic evidence for auxin involvement in arbuscular mycorrhiza initiation
Persistent URL
Author(s)
Hanlon, Meredith T.
Coenen, Catharina
Date Issued
November 2010
Abstract
Formation of arbuscular mycorrhiza (AM) is controlled by a host of small, diffusible signaling molecules, including phytohormones. To test the hypothesis that the plant hormone auxin controls mycorrhiza development, we assessed mycorrhiza formation in two mutants of tomato (Solanum lycopersicum): diageotropica (dgt), an auxin‐resistant mutant, and polycotyledon (pct), a mutant with hyperactive polar auxin transport.
Mutant and wild‐type (WT) roots were inoculated with spores of the AM fungus Glomus intraradices. Presymbiotic root–fungus interactions were observed in root organ culture (ROC) and internal fungal colonization was quantified both in ROC and in intact seedlings.
In ROC, G. intraradices stimulated presymbiotic root branching in pct but not in dgt roots. pct roots stimulated production of hyphal fans indicative of appressorium formation and were colonized more rapidly than WT roots. By contrast, approaching hyphae reversed direction to grow away from cultured dgt roots and failed to colonize them. In intact seedlings, pct and dgt roots were colonized poorly, but development of hyphae, arbuscules, and vesicles was morphologically normal within roots of both mutants.
We conclude that auxin signaling within host roots is required for the early stages of AM formation, including during presymbiotic signal exchange.
Journal
New Phytologist
Department
Biochemistry
Citation
Hanlon, M.T. and Coenen, C. (2010). Genetic evidence for auxin involvement in arbuscular mycorrhiza initiation. New Phytologist, 189(3): 701-709. doi: 10.1111/j.1469-8137.2010.03567.x
Publisher
Wiley Online Library
Version of Article
Published article
DOI
10.1111/j.1469-8137.2010.03567.x
ISSN
0028-646X
e1469-8137
Rights
This is the pre-peer reviewed version of the following article: Hanlon, M.T. and Coenen, C. (2010). Genetic evidence for auxin involvement in arbuscular mycorrhiza initiation. New Phytologist, 189(3): 701-709. doi: 10.1111/j.1469-8137.2010.03567.x, which has been published in final form at https://doi.org/10.1111/j.1469-8137.2010.03567.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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